For example, Song et al investigated the MRI changes before and after radiotherapy in 47 PZ tumours and 12 TZ tumours where the apparent diffusion coefficient changes decreased in both zones after treatment.37 An earlier study by Amico et al reported TZ tumours based on clinical criteria and not MRI scans may benefit from radiotherapy despite having a high PSA level (>20 ng/mL).38 With advances in radiological imaging, tumour location could be mapped before treatment. This evidence concerns the gene KLK3 and neoplasm.