As highlighted in figure 1A, 16 proteins in the intrinsic apoptotic pathway (SMAC, XIAP, APAF1, procaspase-9, procaspase-3, MCL-1, BCL-XL, BCL2, BAX, BAK) and extrinsic apoptotic pathway (FADD, procaspase-3, procaspase-8, FLIP, RIP3, XIAP, CIAP1, BID) underwent multiplexed staining, imaging and single cell analysis on tumor tissue microarray (TMA) cores from each patient.19 Here, APAF1 is linked to neoplasm.