Therefore, in order to explore the mechanism of action and protective effect of sufentanil on acute lung injury in sepsis, this study established a mouse CLP model to study the potential molecular mechanism of sufentanil’s inflammation and apoptosis related to the JAK2-STAT3 signaling pathway, hoping to provide potential targets and new research ideas for the treatment of acute lung injury in sepsis. Here, JAK2 is linked to Sepsis.