To address whether KMT9 regulates DLAT methylation in PCa in mice, we utilized multiplexed immunofluorescence staining to compare distribution and expression of Kmt9α as well as levels of DLAT K596me1 in prostate tissue from Ctrl, Pten/Smad4 KO, and Pten/Smad4/Kmt9α KO mice. Here, PTEN is linked to posterior cortical atrophy.