H2BC21 and neoplasm: To better understand how DKK1 promotes tumor progression in vivo, we performed bulk RNA sequencing using GFP-H2B-mApple-Thy1.1+ PyMT-BO1 cells isolated from orthotopic tumors in WT mice receiving IgG or mDKN01, after exclusion of Ter119+ erythrocytes and CD45+ immune cells (Fig. 4A, Supplementary Fig. 4F).