To investigate whether CAF and bone-derived DKK1 exert similar NK suppressive effects, we co-cultured different numbers of CAFs or osteoblast precursors (pre-OBs), as a source of bone-derived DKK1, together with NK cells and CTV-labeled PyMT tumor cells (2:1 effector to target ratio) and assessed their ability to kill the tumor cells with or without mDKN01. The gene discussed is DKK1; the disease is neoplasm.