DKK1 and neoplasm: To further investigate the role of DKK1 during tumor dissemination, we injected the firefly luciferase-conjugated PyMT-BO1 cell line into albino C57BL/6 mice either intracardiacally (i.c.)to study dissemination to various organs including the bone, or directly into the tibias (i.t.)to study tumor growth in bone, followed by treatment with mDKN01.