CD4 and neoplasm: Although the total macrophages in the tumor were similar in each group, and splenic macrophages were reduced in mice transferred with CD4+ T cells, a close analysis of intratumoral macrophage subsets revealed that the frequency of MHCII− CD206+ M2 macrophages was significantly increased in mice receiving effector CD4+ T cells compared to the no transfer controls, while the M1 subset did not differ substantially (Supplementary Fig. 6g).