To further demonstrate the importance of tumor MHCII in mediating the anti-tumor activity of CD4+ T cells in vivo, we purified CD4+ T cells isolated from brain tumors of saline- or M002-treated mice and transferred them at the same number into MHCII KO (MHCII−/−) mice that were implanted with GSC005 tumor cells at 10 days earlier (Supplementary Fig. 6k). Here, CD4 is linked to brain neoplasm.