To validate whether HoxBlinc lncRNA is required for the NUP98-PHF23 fusion oncoprotein–driven homeotic gene signature and leukemogenesis, we knocked out the promoter region of HoxBlinc lncRNA using sgRNA-targeted CRISPR editing in two NUP98-PHF23–transformed B-ALL primary cells, 961C and A1929, and confirmed this KO by Sanger sequencing (Supplemental Figure 3, A and B). This evidence concerns the gene PHF23 and acute lymphoblastic leukemia.