Molecular profiling of a cellular sample of pleural effusion resulted in negative findings for epidermal growth factor (EGFR), anaplastic lymphoma kinase (ALK), and proto-oncogene tyrosine-protein kinase 1 (ROS-1); the programmed cell death-ligand 1 (PD-L1) tumor proportion score (TPS) on this sample was between 1% and 49%; RET fusion was not assessed on the tissue sample at this stage. The gene discussed is CD274; the disease is neoplasm.