Thus, the aim of the study was to analyze the association of benign unconjugated hyperbilirubinemia with major mutations rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of HFE gene, rs113993960 (ΔF508) of CFTR gene, and rs28929474 (PIZ), rs17580 (PIS) of SERPINA1 gene. This evidence concerns the gene HFE and Hyperbilirubinemia.