CD4 and neoplasm: Although a modest decrease in the absolute number of CD4+ T cells per mg tumor was observed at this timepoint (407.9 ± 34.3 vs. 190.9 ± 58.3; P = 0.024), we observed no changes in the absolute number of CD8+ T cells, Tregs, or neoantigen-specific CD8+ T cells per mg tumor (Supplementary Fig. S2B).