We then examined the precise phenotype of these circulating liver-autoreactive CD4 T cells by spectral flow cytometry using PBMCs from patients affected by different liver diseases: five control non-autoimmune patients with non-alcoholic steatohepatitis (NASH), fourteen AIH patients with an active disease, and thirteen AIH patients in remission under treatment (Supplementary Table 1). Here, CD4 is linked to autoimmune hepatitis.