The TNF-Tg mice established the RA model and were bred as heterozygotes on a C57BL/6 background, then Near infrared-indocyanine green (NIR-ICG) was injected into footpadsMouse Primary Lymphatic Endothelial Cells/C57-6,092 were stimulated by TNF-α and NG-R1. The gene discussed is RTN4R; the disease is rheumatoid arthritis.