In vivo: Intracranial injection of U87, U87-RR (radioresistant U87 cells), and U87-RR/shHSP90B1 (HSP90B1-knockdown U87-RR cells) expressing luciferase.In vitro: Three GBM cell lines, namely U251, U87, and LN18, along with human embryonic kidney 293T cells and glioma stem cells (GSCs) MGG8. This evidence concerns the gene HSP90B1 and glioma.