Similarly, culturing AML cells with CD18KODNT or SCRDNT with AML cells in the top well and CD8+ T cells and AML cells in the bottom well, as done in Fig. 2G, resulted in comparable increase in the cytotoxicity of CD8+ T cells against AML cells in the bottom well (Figure S9B), These results suggest that while CD18 is an important molecule for GvHD suppression, it does not affect the ability of DNTs to promote the GvL activity of CD8+ T cells. This evidence concerns the gene CD8A and acute myeloid leukemia.