CD8A and acute myeloid leukemia: To elucidate the mechanism of how DNTs promote the anti-leukemic activity of Tconv cells, we first assessed whether this phenomenon is through a contact-dependent or -independent mechanism using transwell assays as described in Fig. 2A. Culturing DNT or AML cells in the top well did not affect the viability of AML cells cultured with CD8+ Tconv cells in the bottom well.