To further confirm the role of Siah2 in the activation of mitophagic machinery during ischemia and the consequent induction of mitochondrial biogenesis in the reoxygenation phase, small Siah2 interfering RNAs (siRNA) targeting distinct segments of Siah2 were transiently transfected in cortical neurons before OGD exposure. The gene discussed is SIAH2; the disease is ischemia.