In our study, KEGG pathway analysis indicated that higher concentrations of Thymoquinone (TQ) (50 μM) significantly downregulated genes in the PI3K-Akt signaling pathway in A172 glioblastoma cells, involving 18 differentially expressed genes like LPAR2, FGF10, COL9A3, PDGFB, IL2RB, IL7, PDGFRB, ITGB6, PPP2R2B, COL1A2, THBS3, PDGFD, GNG7, ERBB4, COL4A5, LAMA2, COL6A6, ITGA1. This evidence concerns the gene COL9A3 and glioblastoma.