To investigate their impact on CRC proliferation, we devised distinct formulations of growth factors for CRC culture media, comprising RSPO1, IL6, and Gremlin 1 (RIG); RSPO1, Noggin and EGF (RNE); and established additional experimental groups: RSPO1 and IL6 (RI); RSPO1 and Gremlin 1 (RG); and IL6 and Gremlin 1 (IG); and RSPO1 (R). The gene discussed is RSPO1; the disease is colorectal carcinoma.