Here, we have developed a human 3D neural culture model using iPSC-derived NSC from healthy individuals and FAD patients with PSEN1 mutations-L286V, M146L or A246E, maintained for 12 weeks post-3D plating in vitro. Our model shows early signs of neuronal maturation in the 3D cultures, increased total Tau in 3D-AD when compared to the 3D controls, and early signs of Tau insolubility from cells derived from iPSCs from patients with PSEN1 mutations. This evidence concerns the gene MAPT and Alzheimer disease.