To test the causality between IGF2BP2 expression and NASH, C57BL/6 mice were fed a normal diet or HFHC diet for 28 weeks and then administered via tail vein with adenoviral shRNA targeting the Igf2bp2 gene or negative control, respectively (Fig. 3a). The gene discussed is IGF2BP2; the disease is metabolic dysfunction-associated steatohepatitis.