MAT2A and glioblastoma: As expected, MAT2A was up-regulated in tumorspheres from both U87-MG and U251-MG cell lines that were stimulated by hypoxia, but the upregulation of stemness marker OCT4 and H3K4me3 was only observed in hypoxia-treated tumorspheres from U87 cell line (Fig. 3G), suggesting the importance of MAT2A in tumorsphere formation under hypoxia and that there may be an intrinsic difference in regulatory mechanism between these two GBM cell lines.