Through dimensionality reduction clustering analysis and cell type annotation of LGG single-cell data (Figure 2A), we successfully identified five major cell subpopulations: SOX2 and OLIG2-expressing LGG tumor cells, CD68, AIF1, and FOLR2-expressing macrophages, MBP-expressing oligodendrocytes, PDGFRB-expressing pericytes, and CCL5, CD3E, CD8A, and CD44-expressing T cells. Here, AIF1 is linked to neoplasm.