Furthermore, while double knockdown of SHOC2 and PTEN in cultured liver cancer cells did moderately affect the pERK1/2 and PTEN signals (Fig. 2C and 2D), it was much minor than what was seen in the in vivo model in which double KO of Shoc2 and Pten significantly activated the mTorc1, but without affecting pErk1/2 (Fig. 5C). This evidence concerns the gene PTEN and liver cancer.