The result demonstrated that knockout of Bmal1 in osteoblasts increased the number of osteoblasts and promoted the expression of bone formation markers, leading to elevated trabecular osteoblast activity, which are related to kyphoscoliosis and malformed intervertebral disk in aged mice.53 In another research, Takarada et al. generated osteoblast-specific Bmal1 knockout mice (Bmal1osx-/- mice) by crossing Bmal1flox/flox mice with Osx–Cre transgenic mice. Here, BMAL1 is linked to Kyphoscoliosis.