Pseudo-time ordering predicted a single-lineage trajectory (Fig. 5a), which is consistent with the subcluster enrichment along the tumor-margin-liver axis (Fig. 4e), suggesting that intratumor CAF-FAP and marginal CAF-C7 may arise from HSC at the distal liver, consistent with previous reports in pre-clinical models in HCC53,61. The gene discussed is FAP; the disease is neoplasm.