eIF4E co-IP assays confirmed these findings, with DT-061 inducing a marked increase in 4E-BP1–eIF4E and 4E-BP2–eIF4E interaction, accompanied by a decrease in eIF4E-bound eIF4G in CRC, EC, and PDAC cell lines (Figure 6C and data not shown). Here, EIF4G1 is linked to colorectal carcinoma.