Finally, the results indicate that the iron deficiency is driven by hepcidin accumulation in blood due to decreased GFR (Figure 5).There were several reports in which anemia has been demonstrated in RAS-deficient mice including ACE knockout [4], Agt-KO [5], renin knockout [5,12], and double AT1aR/AT1bR [5] receptor knockout. The gene discussed is ACE; the disease is anemia (phenotype).