To investigate the influence on tumor progression through the YAP O‐GlcNAcylation and subsequently EIF3H guided stabilization, we established EIF3H knockdown (shEIF3H) in 4T1 and EIF3H knockout (EIF3H KO) in MDA‐MB‐231, using cells with an empty vector as the control group. This evidence concerns the gene YAP1 and neoplasm.