Similar to the characterisation of EVs from human pancreatic cancer cell lines, Alix, TSG101 and CD9 were detected whereas calreticulin and GAPDH were undetected in the P100 fractions from KPC and TPAC cells after ultracentrifugation (Fig. 3b, Supplementary Fig. 7). This evidence concerns the gene GAPDH and familial pancreatic carcinoma.