Similar to the characterisation of EVs from human pancreatic cancer cell lines, Alix, TSG101 and CD9 were detected whereas calreticulin and GAPDH were undetected in the P100 fractions from KPC and TPAC cells after ultracentrifugation (Fig. 3b, Supplementary Fig. 7). The gene discussed is CALR; the disease is familial pancreatic carcinoma.