To test the effect of pifithrin-μ and sorafenib on mTOR-activated liver cancer development, we bred Tsc2-floxed mice with Alb-Cre transgenic mice to generate Tsc2flox/flox; Albcre mice (a.k.a. Tsc2−/−) (Fig. S7A, B). Here, TSC2 is linked to liver cancer.