To further investigate the impact of SOD1 mutant MNs on ALS NMJ cytopathies, we cocultured SOD1G85G and SOD1G85R MNs with healthy SOD1G85G SKMs for 7 days (totaling 12 days following myoblast thawing) to establish mature NMJs capable of responding to calcium-induced myotube contractions. The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.