CP and infection: Firstly, to test whether the TRV-based vector can effectively induce AmPDS silencing in JHK, the TRV CP mRNA in the treated JHK leaves was detected by PCR, the results showed that no band was seen in CK, whereas pTRV1 + pTRV2 and pTRV1 + pTRV2 − AmPDS had a clear band (Figure 4A), thus indicating that the vacuum infiltration method was successful in achieving infection of JHK leaves.