TGFB1 and hepatocellular carcinoma: The human recombinant TGF-β1 protein added to the culture of human liver carcinoma HepG2 cells at the concentration of 10 ng/mL with daily media exchange, along with everyday TGF-β1 supplementation, triggers appearing predominantly detached, rounded cell clusters after 3–4 days and almost complete loss of cell adherence to the dish surface up to 7 days with no easily noticeable, apparent changes in morphology before detachment, when compared to the non-treated culture (our unpublished observations).