INS and type 1 diabetes mellitus: It was also found that the proportion of cells positive for only glucagon (glucagon+ cells) was decreased in insulin-deficient islets comparing to insulin-containing islets of control subjects and children with recent-onset T1D (Table 3, Figure 9f), but these differences were possibly related to the small number of glucagon+ cells and higher content of glucagon+/insulin+ cells in insulin-deficient islets.