INS and type 1 diabetes mellitus: A high negative correlation between the proportion of insulin+ cells and glucagon+/insulin+ cells was observed in insulin-containing islets of both control subjects (Figure 10a) and children with recent-onset T1D (Figure 10b), confirming that within the islet, a decrease in the proportion of insulin+ cells may be accompanied by an increase in the proportion of glucagon+/insulin+ cells and vice versa.