HK2 and malignant colon neoplasm: Thus, HK2 phosphorylation by AKT2 at the threonine 473 residue (T473) promotes its binding to mitochondria, which increases HK2 catalytic activity and enhances glycolysis, promoting tumorigenesis in colon cancer cells and lung metastasis in nude mice through upregulation of nuclear factor-kB (NF-kB), hypoxia-inducible factor 1α (HIF1α), matrix metalloproteinase 2 (MMP2), and matrix metalloproteinase 9 (MMP9) upregulation [176].