There is also an in vivo study by Tremblay et al. [68] showing that a high-fat diet increases insulin resistance by inhibition of glucose uptake and glycogen synthesis via overactivation of mTORC1 and inhibition of phosphorylation at the serine site of insulin receptor substrate 1 (IRS-1 Ser312 and Ser636 sites). The gene discussed is IRS1; the disease is Insulin resistance.