This method focused on collecting mutations in several key genes associated with non-hypermutated colon tumors, including APC, TTN, TP53, KRAS, MUC16, MUC5B, PIK3CA, BRAF, SOX9, RYR1, RYR2, RYR3, FBXW7, ARID1A, COL5A1, COL6A3, KIAA019, and PCDH17 for all locations cumulatively, and for the distinct locations one by one. The gene discussed is RYR1; the disease is colonic neoplasm.