MYC and head and neck squamous cell carcinoma: To examine whether autophagy plays a role in MYC-induced changes in trametinib’s effects in HNSCC, JHU22-LV and JHU22-MYC cells were treated with either a vehicle or trametinib (200 nM) for 48 h, followed by a Western blot analysis to assess the expression levels of autophagy-related proteins LC3b and p62.