PPIB and thalassemia: For families 2, 4, 5, 6, 8, 12, 15, and 16, where the fetus inherited the wild-type paternal allele and therefore was predicted to be non-affected, either as β-thalassaemia carrier or with absence of the β-thalassaemia-associated allele, the need for invasive prenatal diagnosis was avoided (Table S8).