To examine the effects of TSPX in NSCLC cells, A549 lung adenocarcinoma cells and SK-MES-1 lung squamous cell carcinoma cells were transduced with the tet-ON lentiviral vector system expressing EGFP and TSPX under control of doxycycline (Dox) (Figure 2A). Here, TSPYL2 is linked to squamous cell lung carcinoma.