An immunofluorescent analysis showed that the overexpressed TSPX was primarily localized in the nuclei in the A549-tetON-TSPX cells, similarly observed in other cell lines, including prostate cancer LNCaP, cervical cancer HeLa, and osteosarcoma U2OS cells (Figure 2C, red color) [16,43,44]. Here, TSPYL2 is linked to osteosarcoma.