AR and its pioneering cofactor FOXA1 have been shown to interact with each other on the chromatin in prostate cancer cells [9], and we confirm a direct physical interaction of NKX3.1 with both AR and FOXA1 through co-immunoprecipitation in LNCaP (Figure 1C) and CWR-R1 (Figure 1D) cells, as well as through a Nano-BiT split luciferase (Promega) interaction system (Figure 1E). This evidence concerns the gene NKX3-1 and prostate cancer.