The inhibitory effects of extracellular regucalcin on cell growth were not attenuated through culture with various signaling inhibitors, including tumor necrosis factor-α (TNF-α), Bay K 8644, PD98059, staurosporine, wortmannin, 5,6-dichloro-1-β-D-ribofuranosyl benzimidazole (DRB), and gemcitabine, which suppressed liver cancer cell proliferation. This evidence concerns the gene TNF and liver cancer.