INS and type 1 diabetes mellitus: Furthermore, immunohistochemical analysis using antibodies to insulin revealed significant loss of insulin secretion (p < 0.001), which confirmed the development of T1DM in mice of this group (Figure 10B(5)): in preparations of the pancreas of mice from the control group, the proportion of insulin-positive cells was 62.8 ± 0.19%; whereas in NOD mice receiving olivetol, the number of insulin-positive cells was 76.1 ± 0.23%.