In the breast cancer study GSE213474 [28], MCF7 cells treated with IFN-γ stimulation showed SPP1 to be significantly downregulated in samples (log2FC = −2.067, p-value = 0.038), when the treatment was applied for a longer duration (48 h v/s 24 h) (Figure 2A). This evidence concerns the gene SPP1 and breast cancer.