In our study, the cafeteria-style diet increased body fat content, and if we also consider that in a state of obesity it has been reported that BDNF levels decrease [41], it is possible that by antagonizing the trkB receptor with ANA-12, hyperinsulinemia was enhanced by the activity of NGF binding to trkA receptor. The gene discussed is NGF; the disease is obesity due to melanocortin 4 receptor deficiency.