To assess AV457 selectivity over a wider dose range and in a different system, we analyzed S6 and AKT phosphorylation in PC3 (prostate cancer 3) cells, a human cell line derived from a prostate adenocarcinoma, which has been utilized previously to assess the selectivity of rapalogs (Schreiber et al., 2019). Here, AKT1 is linked to Familial prostate cancer.