Then, we further performed a replication sample MR analysis, which successfully verified the causality between GDNF (IVW; OR = 1.15; 95% CI: 1.01–1.30; P = 3.53 × 10-2), IL-2RB (IVW; OR = 0.85; 95% CI: 0.72–1.00; P = 4.81 × 10-2), and TRAIL (IVW; OR = 0.91; 95% CI: 0.83–0.99; P = 4.87 × 10-2) with CRC. This evidence concerns the gene GDNF and colorectal carcinoma.