Next, we performed co‐localization analysis of AIM2 with NeuN (a neuronal marker), GFAP (an astrocyte marker), Iba1 (a microglial marker), and CD31 (an endothelial cell marker) in the penumbral area at days 7 and 14 after cerebral ischemia. The gene discussed is AIM2; the disease is brain ischemia.