Lcn2 has been associated with neuroinflammation and glial reactivity in neurodegenerative diseases, our previous research found that neuronal A1R signaling promotes the release of Lcn2, leading to the reactivity of astrocytes.[23] The Notch signaling pathway mediated by Asph and Mfap5 is considered to be closely associated with cell differentiation, fate determination, and proliferation.[46, 47] In HSD mice, the upregulation of Mfap5 is also significant. This evidence concerns the gene ASPH and neurodegenerative disease.