LGALS1 and neoplasm: Following this, we incubated BMDMs in the CM from shGal1 or shNC cells, respectively, for 24 hours, and the results demonstrated that knockdown of Gal1 in tumor cells contributed to the inhibition of PI3K, AKT, and P65 phosphorylation in macrophages, as well as the hindrance of P65 nuclear translocation (Figure 5B,C).