HIV-1WT infection was potently inhibited (~100-fold) by TRIM-BP2Cyp and TRIM-BP2CypK3129H and was not rescued by CsA addition as expected, while TRIM-BP2CypD3126N and TRIM-BP2CypD3126N/K3129H were inactive against HIV-1WT. Here, TRAT1 is linked to infection.