To investigate the role of the RANBP2-Cyp domain in HIV-1 infection and MX2 sensitivity, control and RANBP2∆Cyp cell lines were transduced with a lentiviral vector for doxycycline-inducible expression of MX2 and infected with GFP reporter viruses in the presence or absence of the CypA inhibitor cyclosporine A (CsA) at the time of infection (Fig. 2). The gene discussed is MX2; the disease is HIV-1 infection.